Antibiotic Resistance Pattern of Some Pathogens Isolated From Clinical Samples

At present scenario, the multi drug resistant microorganisms (MDRO’s) causes various life threatening infections which even leads to death of an infected individual. A broad range of microorganisms, such as Staphylococcus aureus and Pseudomonas aeruginosa as well as Enterobacteriaceae like Escherichia coli and Klebsiella pneumoniae were highly resistant to commonly used antibiotics. Resistance towards antimicrobial agents is increasing gradually, with reports of multi drug resistant isolates. The present study was conducted to determine the resistant pattern among the isolates of Escherichia coli and Klebsiella pneumoniae. In present investigation, total 87 urine samples were screened on different bacteriological media. Out of total, 66.67% samples were found positive as well as the ratio of female to male was found higher. Total 116 isolates of E. coli and 62 isolates of K. pneumoniae were found. Antimicrobial susceptibility testing was done as per the method suggested by Kirby-Bauer using disc diffusion technique. Most of the isolates (61.23%) were found resistant when tested against different antibiotics. Out of total resistant isolates, 61.46% isolates belongs to E. coli while remaining were K. pneumoniae. The result of antibiogram showed that, E. coli isolates were highly resistant to cephalothin followed by cefpodoxime, ampicillin, amikacin, amoxyclav, cefepime, ceftriaxone, gentamicin, tetracycline and ciprofloxacin while against cefoperazone/sulbactum and ceftazidime/clavulanic acid less resistance was observed. High resistance among isolates of K. pneumoniae was observed against cefpodoxime, followed by cephalothin, amikacin, ampicillin, amoxyclav, tetracycline, cefepime, ceftriaxone, gentamicin, cefperazone/sulbactum, ciprofloxacin and ceftazidime/clavulanic acid respectively. Imipenem was the most active antibiotic against all isolates of E. coli and K. pneumoniae.